Recent DNA studies have shown that Carolina Dogs lack specific markers indicating they're of European origin, making them indigenous to Native America - at least, since thousands of years ago when they may have crossed the Bering Land Bridge from Asia.

Carolina Dogs go into heat just once a year (like wolves) instead of twice a year like most dogs.

Carolina Dogs cover their excrement by pushing sand over it with their noses. All other dogs outside of the Australian Dingo and an ancient Korean dog use their hindlegs.